t175 tissue culture flask (Sarstedt)

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Sarstedt t175 tissue culture flask
T175 Tissue Culture Flask, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t175+tissue+culture+flasks/flasks+t75/pm41760655-397-12-16
Average 86 stars, based on 1 article reviews

t175 tissue culture flask - by Bioz Stars, 2026-09

86/100 stars

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Cell Culture:

Article Title: NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumors
Article Snippet: .. All cell lines were cultured in T175 tissue culture flasks (Sarstedt, 833912002), passaged every two days, and routinely tested for mycoplasma, with negative results. .. Adherent cell lines were passaged as follows: cell culture vessel, typically a T175 tissue culture flask (Sarstedt, 833912002), was washed once with 6 mL warm 1x phosphate buffer saline (PBS) and then dissociated using 3 mL TrypLE Express (Thermofisher, 12604021) for a few minutes at 37°C until cells started to float.

Article Title: NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumors.
Article Snippet: .. All cell lines were cultured in T175 tissue culture flasks (Sarstedt, 833912002), passaged every two days, and routinely tested for mycoplasma, with negative results. .. AR TI CL E IN P RE SS Cell line passaging and harvest Adherent cell lines were passaged as follows: cell culture vessel, typically a T175 tissue culture flask (Sarstedt, 833912002), was washed once with 6mL warm 1x phosphate buffer saline (PBS) and then dissociated using 3mL TrypLE Express (Thermofisher, 12604021)for a few minutes at 37°Cuntil cells started to float.

Incubation:

Article Title: Conditioned Medium from H 2 O 2 -Preconditioned Human Adipose-Derived Stem Cells Ameliorates UVB-Induced Damage to Human Dermal Fibroblasts.
Article Snippet: .. To obtain HC016-CM, HC016 cells were grown in T175 (Sarstedt, Nümbrecht, Germany) tissue culture flasks to 80% confluence, washed three times with 1× PBS and incubated for 48 h in FBS-free DMEM GlutamaxTM. .. Then, the CM was centrifuged for 5 min at 250× g, filtered through a 0.2-μm filter (Filtropur S 0.2, Sarstedt, Nümbrecht, Germany) and stored at −20 ◦C until use.

other:

Article Title: Hydrogen Peroxide-Preconditioned Human Adipose-Derived Stem Cells Enhance the Recovery of Oligodendrocyte-Like Cells after Oxidative Stress-Induced Damage
Article Snippet: To obtain hASC or HC016 cell CM, cells were grown in T175 (Sarstedt, Nümbrecht, Germany) or T636 (Corning, Tewksbury, MA, USA) tissue culture flasks to 80% confluence, washed three times with 1x PBS and incubated for 48 h in DMEM GlutamaxTM FBS free.

Recombinant:

Article Title: Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study
Article Snippet: .. To prepare vaccine stocks, recombinant MVA viruses were amplified on CEF cell monolayers grown in T175 tissue culture flasks (Sarstedt, Nümbrecht, Germany), purified by ultracentrifugation through 36% sucrose (Sigma-Aldrich, Taufkirchen, Germany) cushion, and reconstituted to high-titer vaccine stock preparation in Tris-buffer saline (pH 7.4) (PanReac AppliChem, Darmstadt, Germany). ..

Amplification:

Purification:

Saline:

Sterility:

Article Title: Trichinella spiralis extracellular vesicles induce anti-inflammatory and regulatory immune responses in vitro.
Article Snippet: The helminth Trichinella spiralis, through its excretory-secretory (ES L1) products, induces immune regulatory mechanisms that modulate the host's immune response not only to itself, but also to bystander antigens, foreign or self in origin, which can result in the alleviation of inflammatory diseases.. Under the influence of ES L1, dendritic cells (DCs) acquire a tolerogenic phenotype and the capacity to induce Th2 and regulatory responses.. Since ES L1 products represent a complex mixture of proteins and extracellular vesicles (TsEVs) the aim of this study was to investigate the impact of TsEVs, isolated from ES L1 products, on phenotypic and functional characteristics of DCs and to elucidate whether TsEVs could reproduce the immunomodulatory effects of the complete ES L1 product.

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